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6 High performance communications issues If you anticipate high data-rate distributed applications, whether for large-scale data movement or process-to-process communication, then enlist the help of a WAN networking specialist and check and re ne the network bandwidth end-to-end using large packet size test data streams (Lots of problems that can affect distributed application do not show up by pinging with the typical 32 byte packets) Problems are likely between application host and site LAN/WAN gateways, WAN/WAN gateways, and along any path that traverses the commodity Internet Considerable experience exists in the DOE Science Grid in detecting and correcting these types of problems, both in the areas of diagnostics and tuning End-to-end monitoring libraries/toolkits (eg NetLogger [78] and pipechar [79]) are invaluable for application-level distributed debugging.

A new map is created as an object property with all of the original map s regex strings precompiled into Python regular expression objects. Note that flags are set on these regexes to accept whitespace for wildcard matches, match on multiline strings, and ignore case sensitivity. You may or may not want to tweak these flags in your own use. Continuing on to Listing 15-4, you can find the definition of the produce_entries() method, where the filtering actually takes place.

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predamount = COALESCE(statelu.avgamount, defaultlu.avgamount)

NetLogger provides for detailed data path analysis, top-to-bottom (application to NIC) and end-to-end (across the entire network path) and is used extensively in the DOE Grid for this purpose It is also being incorporated into some of the Globus tools (For some dramatic examples of the use of NetLogger to debug performance problem in distributed applications, see References [80 83]).

The formula used back in Figure 2-2 didn t reference a fixed column A because you had no need to replicate across multiple columns, so it didn t make a difference whether or not column A was fixed.

different bandwidths have been considered in 10 where simulation results show that capacity gains from 10 to 70% can be achieved. Highest gains are achieved in the networks with less than 5-MHz spectrum. In these narrowband networks, BCCH reuse improvement has the most signi cant effect and also the performance of the hopping layer can be improved the most.

To group multiple radio buttons, use a control named RadioButtonGroup. This control is a non-visual object and provides a way to group RadioButton controls together so that only one of them can be selected at any given time. The RadioButtonGroup control is assigned an id property. Then each RadioButton joins the group by naming the RadioButtonGroup in its groupName property. This is a group of mutually exclusive RadioButton controls, because they all share the same groupName property:

Listing 4-17: agglib.py additions (Part 3 of 3)

10. Save the Flash movie again. At this point, the Flash movie has a skeleton architecture (a blueprint) for our interactive functionality. Now, let s add some content to each section of the movie.

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Extra pDNA expression vector may be obtained if required by mini-prep preparation and digestion with Eco RI and Xho I restriction enzymes. Similarly, the sodB gene fragment is digested with Eco RI and Xho I restriction enzymes. In both cases, digestion should be to completion and products must be puri ed by agarose gel electrophoresis. After puri cation of the two main fragments from each digestion, the two fragments may be ligated together by means of T4 DNA ligase. In the case of our worked example, the pET pDNA expression vector was selected with the idea of ligating the PCR generated sodB gene fragment into the vector MCS in frame with the 3 terminus of a GST tag (see above), thereby generating a new GST sodB fusion gene. After ligation, the newly recombinant pDNA expression construct should be used to transform CaCl2 competent E. coli cells, or introduced into other appropriate host cells by electroporation. For the transformation of a ligation mixture it is best to use an E. coli strain that has been modi ed so as to be particularly ef cient at taking up pDNA, for example the E. coli DH10 strain. After transformation the cells are encouraged to grow without antibiotic for 1 h at 37 C then spread on agar plates for antibiotic selection over a further 16 h incubation at 37 C.